Neuronal Activity and Connectivity Under Controlled Hypoxia

A collection of experimental data from an in vitro model of the ischemic penumbra, studying neuronal dynamics under controlled hypoxia. It was created by Joost le Feber and published in 2020. The data documents changes in spontaneous activity and network connectivity over periods of hypoxia ranging from short durations to approximately 30 hours.

Use Cases

• Analyze the relationship between hypoxia duration (e.g., 6, 12, 30 hours) and the reversibility of network connectivity changes.
• Model the progression of neuronal activity levels (from initial reduction to eventual silence) as a function of controlled oxygen levels (pO2≈20mmHg).
• Investigate the hypothesis that insufficient neuronal activation, rather than hypoxic depth alone, leads to irreversible functional connection loss after extended low-activity periods.

Strengths

• Data is derived from a controlled experimental model of the ischemic penumbra, allowing for precise manipulation of hypoxia levels and duration.
• The study explicitly tracks neuronal dynamics over a defined temporal progression, with observations spanning from short-term (≈6 hours) to long-term (≈30 hours) hypoxia exposure.
• Dataset is published under a permissive CC0 1.0 public domain license, facilitating unrestricted reuse.

Limitations

• The dataset is based on an in vitro model using cultured cortical neurons, which may not fully capture the complexity of in vivo ischemic penumbra pathophysiology.
• Specific data granularity (e.g., row count, column features, file formats) is not provided in the available metadata, limiting assessment of scope and structure.

Provenance

Source

Dryad digital repository.

Collection Method

Data collected from controlled experiments on networks of cultured cortical neurons exposed to defined levels and durations of hypoxia.

Time Range

Experimental observations cover hypoxia exposures from short periods up to approximately 30 hours.

Freshness

Last updated in June 2020.