Cortical Astrocyte Lysine Acetylation Sites and Toxoplasma gondii Infection Effects

A proteomics study identifies 529 lysine acetylation sites across 304 proteins in rat cortical astrocytes, with 277 sites on 186 proteins being novel. The research also maps acetylome changes in astrocytes infected with the brain parasite Toxoplasma gondii, finding 58 proteins with acetylation levels altered more than twofold. The dataset was generated by Anne Bouchut and published in 2020.

Use Cases

• Identify novel lysine acetylation sites by analyzing the 277 unreported sites on 186 proteins.
• Compare protein acetylation levels between infected and uninfected astrocytes using the 58 proteins with >2-fold change.
• Investigate functional enrichment of acetylated proteins in pathways like RNA processing, metabolism, and chromatin biology.
• Examine host-pathogen interactions by correlating acetylome alterations with T. gondii infection in astrocytes.

Strengths

• Documents 529 specific lysine acetylation sites across 304 proteins.
• Reports 277 novel acetylation sites not previously identified in any cell type.
• Quantifies infection-induced changes, identifying 58 proteins with significant acetylation level shifts.

Limitations

• Sample size and number of biological replicates are not specified.
• Data is derived from a single cell type (cortical astrocytes) from Rattus norvegicus, limiting generalizability.
• The temporal dynamics of acetylation changes during infection are not detailed.

Provenance

Source

Dryad digital repository.

Collection Method

Proteome-wide mass spectrometry analysis of lysine acetylation.

Freshness

Last updated 2020-06-24.