Cytotoxic and Cytostatic Flavonoid Screening with FUCCI Cell Cycle Assay

Encompassing screening results for ten flavonoids using a quantitative FUCCI-based cell cycle assay on HeLa cells. It identifies cytotoxic compounds like apigenin and luteolin, and cytostatic compounds like kaempferol which induced G2 arrest. The data was generated by monitoring red and green fluorescence ratios in live cells treated with anticancer drugs.

Use Cases

• Analyze red fluorescence (RF) and green fluorescence (GF) ratio patterns to classify compounds as cytotoxic or cytostatic.
• Investigate structure-activity relationships by comparing flavonoid backbone types (e.g., flavone vs. flavonol) to cell cycle outcomes like G2 arrest.
• Train a model to predict compound cytotoxicity based on fluorescence time-series data from FUCCI-HeLa cells.
• Screen novel compounds for cell cycle modulation by comparing their RF/GF cycling patterns to the ten reference flavonoids.

Strengths

• Data is derived from a quantitative, real-time cell cycle monitoring system (FUCCI).
• Screening results are provided for ten distinct flavonoid compounds.
• Identifies specific cytotoxic (apigenin, luteolin) and cytostatic (kaempferol) compounds with mechanistic detail (G2 arrest).

Limitations

• Sample size is limited to screening results for only ten flavonoid compounds.
• Data is specific to a single cell line (FUCCI-expressing HeLa cells), limiting generalizability.
• The dataset's temporal recency is unknown; the last update was in June 2020.

Provenance

Source

Dryad, authored by Young-Hyun Go.

Collection Method

Generated via a Fluorescence-Ubiquitin Cell Cycle Indicator (FUCCI) assay on a single clone of FUCCI-HeLa cells, monitoring red and green fluorescence ratios over time.

Freshness

Last updated 2020-06-24.