Single Tumor Cell Biophysical Properties Across Five Cell Lines

437 H460, 410 H446, 442 A549, 415 95D, and 290 95C single tumor cells were measured for three intrinsic biophysical markers. The dataset quantifies specific membrane capacitance, cytoplasm conductivity, and instantaneous Young's modulus for each cell type. It was created by Ke Wang using microfluidic constriction channel aspiration and published in 2020.

Use Cases

• Train a classifier to distinguish the five cell types (H460, H446, A549, 95D, 95C) using specific membrane capacitance, cytoplasm conductivity, and instantaneous Young's modulus as features.
• Analyze correlations between instantaneous Young's modulus and cytoplasm conductivity to investigate mechanical-electrical property relationships in single tumor cells.
• Establish reference distributions for specific membrane capacitance across different cell lines to benchmark future cellular biophysics studies.
• Compare the biophysical property profiles, such as specific membrane capacitance and instantaneous Young's modulus, of adherent cell lines (e.g., A549) post-trypsin treatment.

Strengths

• Provides measurements for 1,994 single cells across five distinct tumor cell lines.
• Reports three key intrinsic biophysical properties (specific membrane capacitance, cytoplasm conductivity, instantaneous Young's modulus) not commonly found together.
• Includes mean and standard deviation (e.g., 2.10±0.38 μF/cm²) for each property per cell line, offering distribution context.
• Data is published under a permissive CC0 1.0 public domain dedication license.

Limitations

• Sample sizes per cell line are unequal, ranging from 290 to 442 cells, which may affect statistical power for some comparisons.
• All cells were measured in suspension after trypsin treatment, which may alter native biophysical properties compared to adherent states.
• The dataset is a static snapshot from a single study in 2020, with no ongoing updates or temporal dimension.

Provenance

Source

Dryad digital repository.

Collection Method

Single cells in suspension were aspirated through a microfluidic constriction channel at 25°C; entry processes and impedance profiles were recorded and translated to the three biophysical properties.

Freshness

Last updated 2020-06-24.