Metabolomic Profiles of Asphyxiated Newborn Pigs Following Resuscitation

125 newborn Noroc pigs were subjected to progressive asphyxia and randomized resuscitation protocols. Plasma and urine samples were collected at baseline, 2 hours, and 4 hours after return of spontaneous circulation for metabolomic analysis via nuclear magnetic resonance spectroscopy. The study aimed to identify biomarkers like lactate and pyruvate for subject characterization and prognosis.

Use Cases

• Analyze baseline plasma hypoxanthine and lipoprotein concentrations to correlate with the duration of hypoxia sustained before asystole.
• Compare plasma and urine metabolomic profiles at 2h and 4h post-ROSC to identify metabolic alterations consistent with hypoxia and acidosis.
• Investigate lactate and pyruvate levels across samples to assess their potential prognostic value for outcomes following perinatal asphyxia.
• Examine metabolic response data across different resuscitation protocols (FiO2 levels, ventilation timing, CC ratios) for differential effects.

Strengths

• Data originates from a controlled experimental study involving 125 newborn pigs, providing a substantial sample size for analysis.
• Metabolomic profiles were generated using nuclear magnetic resonance spectroscopy, a standardized analytical technique.
• Samples were collected at three specific time points (baseline, 2h, 4h post-ROSC), enabling longitudinal assessment of metabolic changes.

Limitations

• The dataset is derived from an animal model (newborn pigs), which may limit direct translation of findings to human neonates.
• The study found no evidence of differential metabolic response to the varied resuscitation protocols, potentially limiting comparative analyses.
• Specific data dimensions like row count, column count, and file formats are not provided in the input, obscuring the dataset's structure and size.

Provenance

Source

Dryad digital repository, authored by Daniel Sachse.

Collection Method

Experimental study: newborn pigs were anesthetized, mechanically ventilated, inflicted with progressive asphyxia, and resuscitated with randomized protocols. Plasma and urine samples were collected and analyzed via NMR spectroscopy.

Time Range

Sample collection occurred at baseline, 2 hours, and 4 hours after return of spontaneous circulation (ROSC).

Freshness

Last updated 2020-06-24.

Geography

Study location is not specified; the subject is newborn Noroc pigs.