Muscle Fiber Thixotropy and Rheopexy Measurements from Sinusoidal Oscillations

Data from a study probing the thixotropic and rheopectic behavior of single permeabilized muscle fibers using sinusoidal length changes. The dataset contains measurements of force response and stiffness under varying oscillation frequencies and chemical treatments with EDTA and blebbistatin. It was generated by David Altman and published in 2020.

Use Cases

• Analyze force response data to model cross-bridge dependent thixotropy in activated muscle fibers.
• Compare stiffness measurements from oscillations above and below 1 Hz to study the transition between thixotropic and rheopectic behavior.
• Investigate the effect of blebbistatin and EDTA treatment data on myosin-actin interactions and fiber stiffness.
• Use length change oscillation frequency data to simulate force-dependent cross-bridge kinetics in a half-sarcomere model.

Strengths

• Data originates from controlled experiments on single permeabilized muscle fibers, providing precise biomechanical measurements.
• Includes experimental conditions with specific chemical inhibitors (blebbistatin, EDTA) to isolate mechanisms.
• Published under the permissive CC0 1.0 license, allowing unrestricted reuse.

Limitations

• The dataset size, row count, and specific file structure are unknown, limiting assessment of statistical power.
• Data is from a specific experimental setup; applicability to in vivo muscle function or other fiber types may be limited.
• No sample data or column descriptions are provided, complicating initial data exploration and analysis.

Provenance

Source

Dryad digital repository.

Collection Method

Experimental data from applying sinusoidal length changes to single permeabilized muscle fibers and measuring force response.

Freshness

Last updated in June 2020.