Veronica officinalis Herbal Product Authentication via DNA Metabarcoding and HPLC-MS

Results from authenticating 16 Veronica officinalis herbal products using DNA metabarcoding and HPLC-MS. DNA metabarcoding detected V. officinalis in 15% of products and V. chamaedrys in 62%, confirming widespread adulteration. The study compares the two analytical methods for detecting species substitution and unreported constituents.

Use Cases

• Compare the detection capabilities of DNA metabarcoding and HPLC-MS for identifying specific Veronica species in herbal products.
• Analyze the frequency of V. chamaedrys adulteration across the 16 tested herbal products to assess market integrity.
• Investigate correlations between phytochemical compound profiles from HPLC-MS and species composition results from DNA metabarcoding.
• Use the authentication results to model the prevalence of label inaccuracies for Veronica officinalis herbal products.

Strengths

• Data is derived from 16 distinct herbal product samples, enabling comparative analysis.
• Results are generated from two complementary analytical methods: DNA metabarcoding and HPLC-MS.
• The study provides specific detection rates, such as V. officinalis found in 15% and V. chamaedrys in 62% of products.

Limitations

• The sample size of 16 products is small, limiting statistical power and generalizability.
• The dataset's temporal and geographic scope for the tested products is not specified.
• The specific data format, columns, and raw measurements are not detailed in the provided input.

Provenance

Source

Dryad digital repository.

Collection Method

Data generated via DNA metabarcoding and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) on 16 herbal products.

Freshness

Data was last updated on 2020-06-30.

Geography

Products are associated with European traditional medicine, mainly central eastern Europe and the Balkans, but specific origins are not detailed.