Leptospira Interrogans Cryo-EM Images and Biomechanical Measurements

538.0 MB of raw and processed cryo-electron microscopy images, optical tweezers measurements, and agar penetration assay results for the pathogenic bacterium Leptospira interrogans. The dataset, uploaded by Keigo Abe, supports a study on the mechanical role of the outer-membrane lipoprotein LipL32. Findings demonstrate how LipL32 stabilizes the bacterial cell envelope, contributing to cellular rigidity and invasiveness.

Use Cases

• Analyze the structure of the periplasmic LipL32 layer based on cryo-electron microscopy images.
• Compare whole-cell stiffness between wild-type and mutant strains based on optical tweezers measurements.
• Assess bacterial invasiveness based on results from agar mesh penetration assays.

Strengths

• Includes raw and processed cryo-EM images, providing data at multiple analysis stages.
• Combines structural imaging (cryo-EM) with functional biomechanical measurements (optical tweezers, penetration assays).
• Dataset size is 538.0 MB, indicating substantial primary data.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect methodological bias inherent to the specific experimental techniques used.

Provenance

Source

figshare

Collection Method

Data was generated from cryo-electron microscopy, optical tweezers, and agar mesh penetration assays.

Freshness

Last updated 2026-05-26 00:51:58; freshness should be verified.