Data Set for Optics Express submission: Trade-offs between structural integrity and acquis

A dataset associated with a 2017 Optics Express submission by Jean-François Rupprecht and colleagues. It contains sequences of localization events from labelled tubulin proteins, gold particles, and functionalized silane coverslips. The data was used to analyze trade-offs in stochastic super-resolution microscopy techniques.

Use Cases

• Benchmarking localization algorithms based on sequences of localization events from labelled tubulin.
• Analyzing signal-to-noise trade-offs in super-resolution techniques using data from Alexa Fluor and tdEos fluorophores.
• Studying background noise and fiducial markers using localization sequences of gold particles.
• Evaluating sample preparation and surface effects with localization data from functionalized silane coverslips.

Strengths

• Data is directly associated with a peer-reviewed scientific submission to Optics Express.
• Provides comparative data from multiple experimental conditions: two fluorophore types (Alexa Fluor and tdEos), gold particles, and silane coverslips.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and file size are unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Jean-François Rupprecht, Ariadna Martinez-Marrades, Zhen Zhang, Rishita Changede, Pakorn Kanchanawong, and Gilles Tessier

Collection Method

Generated from stochastic super-resolution microscopy experiments.