Data Sheet 1: 25HC Regulation of Macrophage Polarization in Triple-Negative Breast Cancer

A research data sheet from a study investigating the tumor immune microenvironment of triple-negative breast cancer. The study identified the oxysterol metabolite 25-hydroxycholesterol as a key regulator of CD163+ macrophage polarization via an interferon pathway. The data sheet was authored by Hongmei Zheng and is available under a CC-BY-4.0 license.

Use Cases

• Validate computational predictions of immune cell interactions based on multiplex fluorescence immunohistochemistry data
• Study the link between lipid metabolism and immune cell function based on the identified 25HC metabolic signature
• Investigate potential therapeutic targets for triple-negative breast cancer based on the interferon regulatory factor 7–cholesterol-25-hydroxylase–25HC axis
• Benchmark macrophage polarization states in tumor models based on CD163+ cell data

Strengths

• Data is associated with a peer-reviewed research methodology employing multiplex fluorescence immunohistochemistry and bioinformatic analyses.
• The study includes validation through in vitro cellular assays and in vivo mouse models.

Limitations

• The dataset is a single PDF file of 386.5 KB, suggesting limited scope and likely containing summary data rather than raw experimental datasets.
• Column-level documentation and sample data are unavailable, requiring manual inspection after download to understand data structure.

Provenance

Source

figshare

Collection Method

Multiplex fluorescence immunohistochemistry and bioinformatic analysis of tumor samples, validated with cellular and mouse models.

Freshness

Last updated 2026-05-28 04:43:31