Comparison of HDL Proteomic Analysis Methods

25.8 KB of data compares two gold-standard methods for isolating high-density lipoprotein (HDL) from plasma. Jack D. Beazer published this dataset on figshare under a CC-BY-4.0 license, last updated in May 2026. Results show HDL composition and anti-inflammatory function differ significantly between sequential and density gradient ultracentrifugation techniques.

Use Cases

• Compare protein content in HDL fractions isolated by different ultracentrifugation methods based on the described total protein measurements.
• Analyze the detection of key HDL proteins like apolipoprotein A-I and paraoxonase-1 across isolation techniques as described in the results.
• Evaluate the impact of isolation method on HDL subclass distribution analysis based on the described gel electrophoresis results.
• Assess vascular anti-inflammatory function of HDL in relation to isolation technique purity based on the described functional assay results.

Strengths

• Dataset includes specific quantitative results, such as total protein content (10.14 ± 1.49 mg/mL vs. 3.18 ± 1.10 mg/mL) and anti-inflammatory function percentages (77.5% ± 4.4% vs. 58.3% ± 4.8%).
• Clear methodological comparison between two established laboratory techniques (sequential density ultracentrifugation and density gradient ultracentrifugation).
• Published under a permissive CC-BY-4.0 license, allowing for reuse and redistribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (25.8 KB), indicating limited scope and likely a summary of results rather than raw experimental data.

Provenance

Source

figshare

Collection Method

Data likely originates from a laboratory study comparing HDL isolation techniques, analyzed by nLC-MS/MS, ELISA, and gel electrophoresis.

Freshness

Last updated 2026-05-15 05:38:07; freshness should be verified.