CRISPR-Cas13a Assay Results for Epstein-Barr Virus Detection

A research dataset describes the performance of a two-step recombinase-aided amplification and CRISPR/Cas13a assay for detecting Epstein-Barr virus DNA. The dataset likely contains results from fluorescence and lateral flow strip analyses, including detection limits and cross-reactivity tests. The data was published by Hui Liu on figshare in May 2026 under a CC-BY-4.0 license.

Use Cases

• Benchmarking diagnostic assay sensitivity based on the reported limit of detection of 10^1 copies per reaction
• Evaluating visual readout methods for resource-limited settings based on the lateral flow strip analysis results
• Studying cross-reactivity in viral diagnostics based on the reported absence of cross-reactivity against tested control viruses

Strengths

• The assay demonstrated a dynamic range up to 10^7 copies per reaction
• The fluorescence assay achieved a 100% positive detection rate in a preliminary clinical evaluation of 13 samples

Limitations

• The dataset is a single PDF file of 816.5 KB; the underlying tabular data structure is not directly accessible
• Column-level documentation is absent; field semantics must be inferred from the paper
• Row count is unknown, which may limit suitability assessment

Provenance

Source

figshare

Collection Method

Experimental results from a developed nucleic acid detection method.

Freshness

Last updated 2026-05-22 04:30:19