Human-on-Human Assay for Anti-Myocardial Antibodies in Heart Disease

A novel human cell-based assay developed to detect heart-reactive antibodies in patient serum, addressing limitations of traditional primate-based tests. The method uses human cardiomyocytes differentiated from induced pluripotent stem cells (iPSC-CMs) and indirect immunofluorescence staining. The dataset, authored by Anne Auer and last updated in May 2026, is a 835.1 KB PDF describing the protocol.

Use Cases

• Developing diagnostic tools for anti-myocardial antibodies based on the described human-on-human assay.
• Stratifying patients with myocardial disease by immune status using the immunofluorescence detection method.
• Studying cardiomyocyte-specific immunoglobulin reactivity in patient plasma as described in the protocol.

Strengths

• Describes a novel, physiologically relevant human cell-based assay, addressing ethical and cross-reactivity issues of primate-based tests.
• The protocol is designed for use with standard widefield microscopy systems available in clinical labs, suggesting practical accessibility.
• Dataset is openly shared under a CC-BY-4.0 license, permitting reuse and modification.

Limitations

• The dataset is a single 835.1 KB PDF document, which is a very limited data scope.
• Row count and column-level documentation are absent; the file contains a methodological description rather than structured data.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

The description details a laboratory method developed by the author.

Time Range

Publication date suggests the method was developed circa 2026.

Freshness

Last updated 2026-05-08 05:29:34; freshness should be verified.

Geography

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