Ambn-IRESCre Mouse Model Data: Ameloblast-Specific Smad4 Silencing

Data Sheet 1_A novel Ambn-IRESCre mouse line allows ameloblast-specific Smad4 silencing.pdf is a 4.4 MB PDF file published on figshare by Rucha Arun Bapat. It describes the development and validation of a novel Ambn-IRESCre mouse line for studying gene function during enamel formation, specifically used to silence Smad4 in ameloblasts. The dataset was last updated on June 3, 2026, and is shared under a CC-BY-4.0 license.

Use Cases

• Validate ameloblast-specific Cre-recombinase expression based on in situ hybridization and reporter line data.
• Study the role of Smad4 in post-birth enamel formation based on phenotypic analysis of conditional knockout mice.
• Compare the specificity of the Ambn-IRESCre model to the Krt14-Cre driver line for amelogenesis research.
• Analyze enamel defects such as hypomineralization and loss of rod-interrod structure resulting from Smad4 silencing.

Strengths

• Dataset is openly licensed under CC-BY-4.0, allowing for broad reuse.
• The description provides specific experimental details, including the use of mTmG and LacZ reporter lines for validation.
• The research addresses a specific limitation of a previously used model (Krt14-Cre) for studying post-birth events.

Limitations

• The dataset is a single 4.4 MB PDF file, which is a small-scale resource.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Rucha Arun Bapat

Collection Method

Likely contains experimental data from the development and analysis of a novel Ambn-IRESCre mouse line.

Freshness

Last updated 2026-06-03 05:40:11; freshness should be verified.