RPA-CRISPR/Cas12a Assay Data for Rickettsia Rickettsii Detection in Canine Blood

A dataset containing results from a rapid isothermal RPA–CRISPR/Cas12a assay developed to detect Rickettsia rickettsii, the bacterium causing Rocky Mountain spotted fever. The data includes analytical sensitivity results from synthetic DNA standards and specificity testing against non-target pathogens, as well as performance data from archived longitudinal canine whole-blood DNA extracts. The dataset was authored by Sezayi Ozubek and last updated on April 16, 2026.

Use Cases

• Benchmarking the analytical sensitivity of molecular assays based on the reported detection limit of 60–70 copies per reaction.
• Evaluating assay specificity for Rickettsia rickettsii based on cross-reactivity testing results against other spotted fever group rickettsiae.
• Analyzing the intermittent detection patterns of Rickettsia rickettsii in canine whole blood samples over post-infection days.
• Developing rapid molecular workflows for pathogen detection based on the described RPA–Cas12a methodology.

Strengths

• Assay demonstrated no cross-reactivity with a panel of non-target spotted fever group rickettsiae and other tick-borne bacteria.
• Reported analytical detection limit of 60–70 copies per reaction using quantified synthetic DNA standards.
• Data includes results from archived longitudinal canine whole-blood DNA extracts from six experimentally infected dogs.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is very small at 17.6 KB, indicating limited scope.
• Performance in canine blood was described as intermittent and limited to specific post-infection days.

Provenance

Source

figshare

Collection Method

Experimental results from a molecular assay study.

Time Range

The temporal coverage of the experimental data is not specified.

Freshness

Last updated 2026-04-16 05:18:53; freshness should be verified.

Geography

The geographic coverage of the data is not specified.