Proteomic Analysis of Aged Murine T Cells and PDHK1 Expression

Rajkumar S. Kalra published a proteomic dataset on figshare in May 2026. The data supports research into age-related immune decline by identifying pyruvate dehydrogenase kinase 1 (PDHK1) as downregulated in aged mouse T cells. The dataset is 2.3 MB and available under a CC-BY-4.0 license.

Use Cases

• Analyze age-related changes in T cell proteomes based on data-independent acquisition mass spectrometry.
• Investigate the link between glycolytic enzyme expression and T cell activation based on PDHK1 levels.
• Model the impact of PDHK1 manipulation on T cell effector function based on CRISPR-mediated deletion and overexpression experiments.
• Compare proteomic profiles between young and aged T cells based on CD4+ and CD8+ subsets.

Strengths

• Dataset is 2.3 MB, facilitating quick download and initial analysis.
• Proteomic analysis was performed using data-independent acquisition mass spectrometry, a specific methodology.
• Findings are validated across both CD4+ and CD8+ T cell subsets in mice.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is specific to murine T cells, which may limit direct translation to human immunology.

Provenance

Source

figshare

Collection Method

Data-independent acquisition (DIA) mass spectrometry-based proteomic analysis.

Freshness

Last updated 2026-05-11 14:41:02; freshness should be verified.