Alkaline Mineral Complex Effects on Cattle Gut Microbiota and Metabolism

A dataset from a 142-day feeding trial with 54 Simmental crossbred cattle, investigating the effects of alkaline mineral complex (AMC) supplementation on colonic health. The study includes results from serum biochemistry, histology, 16S rRNA gene sequencing, metabolomics, and proteomics analyses. The data was authored by Jia Li and last updated in June 2026.

Use Cases

• Analyze changes in gut microbial alpha-diversity and pathogen abundance based on 16S rRNA sequencing results.
• Investigate alterations in metabolic pathways like sphingolipid metabolism and BCAA degradation based on metabolomic profiles.
• Study the enrichment of host pathways such as aldosterone-regulated sodium reabsorption and tight junction formation based on proteomic data.
• Correlate dietary supplementation with physiological markers like serum ion concentrations, cytokine levels, and fecal consistency scores.

Strengths

• Includes multi-omics data from 16S rRNA sequencing, metabolomics, and proteomics on the same subjects.
• Study design involves a controlled 142-day feeding trial with 54 cattle and multiple sample types (blood, tissue, mucosa, contents).
• Results are supported by statistical significance values (P-values) for key findings like fecal consistency and serum markers.

Limitations

• The dataset is small (276.0 KB), suggesting it may be a summary document rather than the full raw experimental data.
• Column-level documentation is absent; field semantics must be inferred after download.
• Sample sizes for specific analyses vary (e.g., n=3 for histology, n=5 for metabolomics), which may limit statistical power for some comparisons.

Provenance

Source

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Collection Method

Data collected from a controlled animal feeding trial, with subsequent laboratory analyses including sequencing and spectrometry.

Time Range

Covers a 142-day feeding trial period.

Freshness

Last updated 2026-06-02 05:26:47; freshness should be verified.