Budding Yeast ORC ssDNA Binding and Origin Specificity Analysis

A 3.9 MB PDF published by Hironori Kawakami on April 22, 2026, details in vitro and genome-wide analyses of the origin recognition complex (ORC) in budding yeast. The study characterizes how an initiator-specific motif (ISM)-related region in Orc1 limits ORC binding to single-stranded DNA (ssDNA) to promote faithful replication origin selection. Findings suggest base composition-dependent modulation of ORC–ssDNA binding and a functional divergence in eukaryotic ORC to safeguard genome stability.

Use Cases

• Modeling protein-DNA interaction affinities based on described in vitro binding assays.
• Analyzing genome-wide protein binding peak overlaps based on the mentioned ORC- and RPA-enriched regions.
• Investigating the functional conservation of initiator-specific motifs across domains of life as referenced in the description.
• Studying the trade-off between origin-specific binding and ssDNA binding redistribution in vivo.

Strengths

• Published under a permissive CC-BY-4.0 license, allowing for broad reuse.
• Includes both in vitro biochemical and in vivo genetic/chromatin-based analyses as described.
• Focuses on a conserved molecular mechanism (ISM) with implications for understanding eukaryotic replication.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.
• The primary data file is a PDF (3.9 MB), which may require extraction or manual digitization of underlying data.

Provenance

Source

figshare

Collection Method

Likely contains experimental results from in vitro binding assays and genome-wide analyses (ChIP-seq, etc.) as described.

Time Range

null

Freshness

Last updated 2026-04-22 05:35:16; freshness should be verified.

Geography

null