Guinea Pig Laminin Isoform Expression in Allergic Asthma Phenotypes

A research dataset compares laminin isoform expression in airway and vascular smooth muscle, serum, and bronchoalveolar lavage fluid from guinea pigs. The study includes 18 animals divided into three groups: an allergic asthma model, a non-responder phenotype, and a saline control. It correlates specific laminin isoforms with pathophysiological responses like airway obstruction and inflammatory cell counts.

Use Cases

• Correlate ASM expression of LN α1 and LN α2 with measurements of antigen-induced airway obstruction and hyperresponsiveness.
• Differentiate between allergic asthma and non-responder phenotypes using serum levels of LN α1 and LN β2 as potential markers.
• Analyze the role of LN β3 in airway inflammation by correlating its levels in serum and BAL fluid with inflammatory cell counts.
• Compare laminin isoform expression patterns in airway smooth muscle (ASM) versus intrapulmonary vascular smooth muscle (IVSM) across experimental phenotypes.

Strengths

• Experimental design includes three distinct phenotype groups with 6 animals per group for controlled comparison.
• Data integrates multiple measurement types: immunohistochemistry for tissue expression and ELISA for soluble proteins in serum and BAL fluid.
• Findings correlate specific molecular data (laminin isoforms) with functional physiological outcomes like airway obstruction.

Limitations

• Small sample size of 18 total animals limits statistical power and generalizability of findings.
• Data is contained within a single 1.6 MB DOCX file, requiring manual extraction for computational analysis.
• Study is specific to a guinea pig model of ovalbumin sensitization, which may not directly translate to human asthma.

Provenance

Source

Figshare, authored by Ivonne Pacheco-Alba.

Collection Method

Guinea pigs were sensitized and challenged with ovalbumin or saline; laminin expression was evaluated via immunohistochemistry and ELISA.

Time Range

Covers the experimental period of the described study.

Freshness

Last updated in March 2026.