Data Sheet 1_Anti-PD-1 blockade reverses low-intensity electric stimulation-driven pancrea

Lingmin Jiang published a 460.3 KB PDF on figshare in May 2026 detailing research on pancreatic ductal adenocarcinoma (PDAC). The study explores the tumor immune microenvironment following low-intensity electric stimulation (LIES) and evaluates synergistic antitumor effects with anti-PD-1 blockade. Methods include orthotopic mouse models, in vitro assays, flow cytometry, immunofluorescence, and single-cell RNA sequencing.

Use Cases

• Analyzing macrophage polarization effects based on flow cytometry and immunofluorescence data mentioned in the description
• Investigating epithelial-mesenchymal transition (EMT) based on in vitro assays under varying electric fields
• Studying CD8+ T cell activation based on single-cell RNA sequencing results
• Examining JAK2-STAT3 pathway activation and PD-L1 expression based on bulk RNA sequencing analysis

Strengths

• The study utilizes orthotopic PDAC mouse models, a method described as being used for the first time to evaluate immune modulation effects.
• Multiple analytical techniques are employed, including flow cytometry, multicolor immunofluorescence, and single-cell RNA sequencing, suggesting a multi-modal data approach.
• The underlying mechanism was investigated via bulk RNA sequencing and validated in vitro, indicating a layered experimental design.

Limitations

• The dataset is a single PDF file; the underlying raw data (e.g., sequencing files, cytometry data) are not directly provided.
• Column-level documentation is absent; field semantics must be inferred from the research paper.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

Research data generated from orthotopic mouse models, in vitro assays, and sequencing techniques.

Freshness

Last updated 2026-05-19 05:40:19; freshness should be verified.