Modified MSRE-qPCR Method for Cervical Cancer Detection via cfDNA Methylation

A methodological paper describing an enhanced Methylation-sensitive restriction enzyme-quantitative polymerase chain reaction (MSRE-qPCR) assay for detecting cervical cancer. The method was applied to 24 blood samples from cervical cancer patients and 21 matched tissue samples, achieving 87.5% sensitivity and 90.3% specificity. The dataset, authored by Yaru Liu and last updated in May 2026, is shared under a CC-BY-4.0 license.

Use Cases

• Benchmarking methylation detection methods based on the described MSRE-qPCR protocol.
• Developing logistic regression models for cancer status prediction based on the RXFP3-L1, RXFP3-L2, ZNF671, and SOX1 gene panel.
• Assessing assay performance for early cancer detection based on the reported sensitivity and specificity metrics.
• Evaluating techniques for low-input DNA methylation analysis based on the method's requirement of only 1.5 ng of DNA.

Strengths

• Method achieved 87.5% sensitivity and 90.3% specificity for cervical cancer detection.
• Assay requires only 1.5 ng of DNA input, addressing low cfDNA yield limitations.
• A methylation panel of four genes yielded an AUC of 0.917 in the development set.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The 421.8 KB file size suggests the primary content is a methodological document, not a large-scale data table.

Provenance

Source

figshare

Collection Method

Enhanced MSRE-qPCR method applied to patient blood and tissue samples.

Time Range

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Freshness

Last updated 2026-05-04 04:19:05; freshness should be verified.

Geography

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