Biochemical and Functional Characterization of Porphyromonas gingivalis HmuS Protein

A research article detailing the characterization of the HmuS protein in the bacterium Porphyromonas gingivalis. The study, authored by Patryk Cierpisz and published on figshare in May 2026, investigates the protein's role in heme binding and iron metabolism using spectroscopy, HPLC, LC-MS, and RNA-seq methods.

Use Cases

• Understanding bacterial heme metabolism mechanisms based on spectroscopic and chromatographic analyses described.
• Studying gene expression changes related to heme uptake based on RNA-seq and RT-qPCR results mentioned.
• Comparing protein function across bacterial species based on the comparison with Bacteroides fragilis homologous protein.
• Investigating biofilm formation factors based on the observed reduction in the mutant strain.

Strengths

• Detailed methodological description includes UV-visible spectroscopy, HPLC, LC-MS, RNA-seq, RT-qPCR, and Western blotting.
• License is CC-BY-4.0, permitting broad reuse with attribution.
• File is 2.4 MB, ensuring quick download and access.

Limitations

• Dataset is a single PDF document; underlying raw data tables or sequences are not directly provided.
• Row count and column-level documentation are unknown, requiring manual extraction of data from the text.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Experimental laboratory research involving bacterial culture, protein analysis, and gene expression assays.

Time Range

Study period not specified; publication date is 2026-05-19.

Freshness

Last updated 2026-05-19 08:46:23; freshness should be verified.

Geography

Geographic origin of the bacterial strain or research institution is not specified.