Eimeria tenella Bioluminescence Assay for Anticoccidial Drug Screening

A genetically modified Eimeria tenella line expressing NanoLuc luciferase enables rapid, quantitative in vitro screening of anticoccidial compounds. The dataset, published by Martina Felici in 2026, details parasite growth measured in relative light units (RLU) under different drug treatments. It includes results for commercial drugs like salinomycin and robenidine, as well as natural compounds such as thyme and oregano essential oils.

Use Cases

• Quantify dose-dependent inhibition of Eimeria tenella replication based on relative light unit (RLU) slopes.
• Compare the efficacy of commercial anticoccidial drugs versus plant-derived compounds based on invasion and replication reduction percentages.
• Optimize in vitro assay conditions for anticoccidial screening based on multiplicity of infection (MOI) and time course analysis.
• Evaluate compound effects on different parasite life cycle phases based on bioluminescence monitoring of schizont formation and merozoite release.

Strengths

• Includes specific quantitative results, such as a replication slope of 2908 RLU/h for the selected MOI 4:1 condition.
• Compares two distinct experimental designs: short pre-incubation of sporozoites and continuous exposure throughout development.
• Reports concrete efficacy metrics, e.g., pre-incubation reducing invasion to 65% for salinomycin and 44% for robenidine.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data file is a 821.3 KB DOCX document, which suggests a limited, potentially narrative-driven data scope.

Provenance

Source

figshare, author Martina Felici

Collection Method

Experimental data from a bioluminescent in vitro assay using a genetically modified Eimeria tenella line (EtNluc).

Freshness

Last updated 2026-03-18 07:42:18