Zebrafish Cardiac Valve Development Study via CRISPR Mutants

A 3.9 MB research document details a study on cardiac valve development using three CRISPR/Cas9-generated zebrafish mutant strains. The work includes cardiac function assessment, transcriptomic profiling via RNA-seq, and validation through whole-mount in situ hybridization and qRT-PCR. It investigates the roles of bmpr2a and bmpr2b genes, identifying ECM-receptor interaction as a key regulatory pathway.

Use Cases

• Compare cardiac contractile dysfunction phenotypes across bmpr2a−/−, bmpr2b−/−, and double-knockout mutant zebrafish strains.
• Analyze RNA-seq data for upregulation of valve-related genes like fn1b, has2, and nfatc1 in mutant strains.
• Validate pathway analysis findings on ECM-receptor interaction as a regulatory axis in BMPR2-mediated development.
• Correlate gene expression changes in cardiac markers myl9a and tnnc1a with valve defects observed at 52 hours post-fertilization.

Strengths

• Study employs three distinct genetically modified zebrafish strains for comparative analysis.
• Validation integrates multiple methods: RNA-seq, whole-mount in situ hybridization, and qRT-PCR.
• Research identifies a specific molecular pathway (ECM-receptor interaction) linked to valve development.

Limitations

• Dataset is a 3.9 MB DOCX file, not a structured, machine-readable table, limiting direct computational analysis.
• Sample size and specific row/column counts for experimental measurements are not provided.
• Findings are specific to zebrafish embryogenesis at 52 hpf, requiring translation for mammalian or human contexts.

Provenance

Source

figshare admin frontiersin

Collection Method

Data generated via CRISPR/Cas9 gene editing, cardiac function assessment (M-mode), and RNA-seq transcriptomic profiling.

Time Range

Observations centered at 52 hours post-fertilization (hpf) in zebrafish embryos.

Freshness

Last updated March 2026.