CRISPR/Cas9 Genome Editing Data for Citrus Canker Resistance and Early Flowering

Data Sheet 1_Cas9/sgRNA-mediated genome editing of citrus via mature tissue transformation enables both high-efficacy genome editing and early flowering.pdf contains experimental results from a CRISPR/Cas9 genome editing study on Valencia sweet orange. The research, authored by Hongge Jia and uploaded on figshare in 2026, tested mature tissue transformation to target the CsLOB1 promoter alleles for canker resistance. It reports mutation frequencies of 100%, 21.43%, and 41.94% across three transgenic lines and notes flowering within 15 months.

Use Cases

• Evaluating mutation efficacy of specific CRISPR/Cas9 constructs based on reported mutation frequencies
• Studying the relationship between promoter allele editing and canker symptom reduction based on infection assay results
• Analyzing the impact of mature tissue transformation on flowering time in citrus based on the reported 15-month flowering window

Strengths

• Reports specific mutation frequencies (100%, 21.43%, 41.94%) for three independent transgenic lines
• Includes a concrete result for flowering time reduction, with edited lines flowering within 15 months
• Explicitly states no off-target mutations were identified for the tested sgRNA

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is small (402.4 KB), suggesting limited scope

Provenance

Source

Hongge Jia

Collection Method

Agrobacterium-mediated transformation of mature citrus stem segments and subsequent molecular analysis.

Freshness

Last updated 2026-05-11 05:29:16; freshness should be verified