Case Report: mNGS Analysis of a Complex Infection in an Immunocompromised Patient

Rossana Scutari published a detailed clinical case report on figshare in May 2026. The report describes the use of next-generation metagenomic sequencing (mNGS) to diagnose pathogens in a man in his 40s with a severe, fatal infection. The document is 18.8 KB in size and is available under a CC-BY-4.0 license.

Use Cases

• Study the application of mNGS for pathogen discovery based on the described analysis of blood, serum, and lymph node samples.
• Analyze co-infections and polymicrobial landscapes in immunocompromised hosts based on the reported detection of multiple bacteria, fungi, and viruses.
• Investigate diagnostic challenges in granulomatous inflammation based on the case where conventional tests for Mycobacterium were negative despite histological evidence.
• Benchmark bioinformatic pipelines for clinical mNGS based on the described workflow using host DNA depletion and Illumina sequencing.

Strengths

• Provides a detailed, real-world case study of mNGS application in a complex clinical scenario.
• Includes specific pathogen findings such as Fusarium pseudograminearum, Mycobacterium canettii, and Ralstonia sp.
• Reports correlative clinical and immunological data, including CD8+ T-cell and NK-cell depletion.

Limitations

• The dataset is a single case report document (18.8 KB DOCX), not a structured data collection suitable for quantitative analysis.
• Row count and column-level data are unknown, limiting suitability for machine learning tasks.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Rossana Scutari via figshare

Collection Method

Clinical case report based on patient diagnostics and mNGS analysis.

Freshness

Last updated 2026-05-05 04:14:59; freshness should be verified.

Geography

Patient was born in North Africa; specific admission location is not stated.