AtsTPS2 Catalytic Mechanism and Transcriptional Regulation in Acorus tatarinowii

A research document details the catalytic mechanism and transcriptional regulation of the sesquiterpene synthase AtsTPS2 in the medicinal plant Acorus tatarinowii. The study, authored by Fayuan Peng and uploaded in April 2026, identifies AtsTPS2 as responsible for germacrene A biosynthesis and links its overexpression to increased mosquito repellency. Functional assays and site-directed mutagenesis data are included, with the document licensed under CC-BY-4.0.

Use Cases

• Analyze catalytic efficiency of terpene synthases based on site-directed mutagenesis results
• Study transcriptional regulation of biosynthetic genes based on MYC transcription factor binding data
• Investigate correlation between terpene production and mosquito repellency based on transient overexpression assays
• Explore genome-wide characterization of TPS gene families based on the described gene cluster analysis

Strengths

• Includes specific quantitative results, such as a 145% yield increase from a K461A mutant and a 2.02-fold product increase from transient overexpression
• Links biochemical function to a practical application, showing mosquito repellency increased to 83%
• Provides detailed methodological context from HS-SPME-GC-MS analysis and genome-wide characterization

Limitations

• The primary data format is a DOCX document (15.6 MB), which likely contains textual analysis and figures rather than structured, machine-readable data tables
• Row count and column-level documentation are unknown, making direct data extraction and analysis challenging
• The dataset's scale is limited to a single study on one plant species

Provenance

Source

figshare

Collection Method

Likely contains compiled results from laboratory experiments including HS-SPME-GC-MS, site-directed mutagenesis, and transient overexpression assays.

Freshness

Last updated 2026-04-29 05:56:52