Data Sheet 1_CD14+CD16+ monocyte-derived TREM2 macrophages promote lung fibrosis in system

Jee Young Kim's research data, last updated May 29, 2026, investigates scar-associated macrophages in lung tissues from patients with systemic sclerosis-interstitial lung disease and healthy controls. The 2.1 MB dataset includes single-cell RNA sequencing profiles, immunofluorescence staining results, and flow cytometry data from in vitro monocyte differentiation experiments. It supports analysis of the GM-CSF–TREM2–TGF-β1 axis driving profibrotic macrophage–fibroblast crosstalk.

Use Cases

• Analyze transcriptional profiles of TREM2 and GPNMB-expressing macrophages based on single-cell RNA sequencing data mentioned in the description.
• Investigate the fibrotic effects of monocyte-derived macrophages on collagen production based on co-culture experiment results.
• Study the functional impact of TREM2 inhibition on macrophage responses based on bulk RNA-seq and protein level assays described.
• Compare macrophage populations and cytokine levels (CCL2, TGF-β1) between patient-derived and healthy control samples based on flow cytometry and ELISA data.

Strengths

• Data is derived from multiple experimental methods including single-cell RNA sequencing, immunofluorescence, flow cytometry, and ELISA, providing multi-modal evidence.
• Includes comparative data from both patients with systemic sclerosis-interstitial lung disease and healthy controls.
• The dataset is associated with a specific mechanistic pathway (GM-CSF–TREM2–TGF-β1 axis) identified in the study.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The dataset is a 2.1 MB DOCX file, suggesting it may contain processed results or summaries rather than raw sequencing data.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

Jee Young Kim via figshare.

Collection Method

Data generated from single-cell RNA sequencing of lung tissues, immunofluorescence staining, flow cytometry, and in vitro cell culture experiments.

Freshness

Last updated 2026-05-29 06:11:33; freshness should be verified.