Data Sheet 1_CD32b: Mouse Dendritic Cell Subsets from Bone Marrow Culture

A research data sheet by Wanho Choi, last updated in May 2026, describes the heterogeneity of mouse dendritic cells generated in vitro. The 10.7 MB PDF details two distinct dendritic cell lineages, CD32b- and CD32b+ GM-DCs, which arise from separate progenitor cells at different time points during bone marrow culture with GM-CSF. The data sheet characterizes their developmental pathways and distinct functional capacities, such as T cell stimulation.

Use Cases

• Classifying dendritic cell subsets based on CD32b expression markers described in the study.
• Modeling the developmental kinetics of GM-CSF-induced dendritic cells from different progenitor lineages.
• Investigating functional differences in immune cell activation, such as CD4+ T cell stimulation capacity, between the identified subsets.

Strengths

• Data is associated with a peer-reviewed research publication, providing methodological context.
• The 10.7 MB file size suggests the PDF contains detailed figures and experimental data.
• The description provides specific biological markers (e.g., CD32b, Ly6C, MHC II) used to distinguish cell populations.

Limitations

• The dataset is a single PDF; underlying raw data tables or flow cytometry files are not directly accessible.
• Row and column counts are unknown, making suitability for quantitative analysis unclear without manual extraction.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Wanho Choi, via figshare.

Collection Method

Data generated from in vitro culture of mouse bone marrow with GM-CSF, followed by flow cytometry and functional assays.

Freshness

Last updated 2026-05-29 04:49:10; freshness should be verified.