Macrophage Phenotype Data from 3D Co-Culture Experiments with Tunable Stiffness

A dataset from a 2026 study published on figshare investigating vascular fibrosis. It contains data on bone-marrow derived macrophages and mouse embryonic fibroblasts co-cultured in three-dimensional collagen scaffolds with storage moduli of approximately 200 Pa and 2000 Pa. The research, authored by Jennessa WX Ng, highlights stiffness-dependent upregulation of CD54 and CD140a markers on macrophages.

Use Cases

• Modeling macrophage-fibroblast crosstalk based on the described 3D co-culture system.
• Investigating stiffness-dependent cell phenotype changes based on the reported upregulation of CD54 and CD140a.
• Analyzing the role of mechanical versus soluble cues in cell signaling based on the confocal imaging results described.

Strengths

• Data is derived from a physiologically relevant 3D culture system, contrasting with non-physiological 2D systems mentioned.
• Experimental conditions include scaffolds with two distinct, quantified storage moduli (~200 Pa and ~2000 Pa).
• Dataset is openly licensed under CC-BY-4.0, facilitating reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 1.7 MB file size suggests a relatively small dataset.

Provenance

Source

figshare

Collection Method

Experimental data from in vitro co-culture studies using bone-marrow derived macrophages and mouse embryonic fibroblasts.

Freshness

Last updated 2026-06-03 04:47:21; freshness should be verified.