Data Sheet 1_Combined use of Bumetanide and MGE cell transplantation alleviates neuropathi

Ninety adult female C57BL/6N mice were used to investigate the combined effect of Bumetanide and MGE cell transplantation on neuropathic pain after spinal cord injury. The dataset, authored by Yang Yu and last updated in March 2026, includes results from behavioral assessments, ELISA, Western blotting, qRT-PCR, and immunofluorescence staining. It provides evidence on mechanisms involving the NF-κB pathway, microglia activation, and GABAergic signaling.

Use Cases

• Modeling the relationship between NKCC1/KCC2 imbalance and pain behavior based on biochemical assay results.
• Analyzing the additive effects of combined drug and cell therapy on glial scarring and neuronal protection based on immunofluorescence and Western blot data.
• Investigating correlations between GABA-A receptor expression and behavioral pain relief based on qRT-PCR and behavioral assessment data.

Strengths

• Data is derived from a controlled study with 90 mice divided into 5 distinct treatment groups.
• Multiple experimental methods (behavioral, ELISA, Western blot, qRT-PCR, immunofluorescence) provide a multi-faceted view of the biological mechanisms.
• Dataset is openly licensed under CC-BY-4.0, permitting reuse and redistribution.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is small in scale at 980.8 KB, indicating limited scope.
• Data may reflect bias inherent to a single animal model study on figshare.

Provenance

Source

Yang Yu via figshare.

Collection Method

Data was generated from a laboratory study involving a T10 moderate spinal cord contusion model in mice, with treatments administered and various assays performed.

Freshness

Last updated 2026-03-18 05:21:21; freshness should be verified.