Comparative Analysis of Interferon Regulatory Factors in Human and Canine Keratinocytes

3.7 MB of experimental data from a study comparing interferon and interferon-stimulated gene expression in human and canine keratinocytes. The dataset, authored by Sarah Quinlan and last updated in May 2026, includes results from CRISPR-Cas9 knockout and shRNA knockdown experiments on IRF1, IRF3, and IRF7. It is shared under a CC-BY-4.0 license on figshare.

Use Cases

• Compare basal and induced type I and type III interferon expression between species based on the described RT-qPCR results.
• Investigate the species-specific and stimulus-specific roles of IRF1, IRF3, and IRF7 based on the knockout/knockdown experimental data.
• Study non-IRF mediated mechanisms regulating interferon responses in keratinocytes as suggested by the conclusions.
• Validate the dog as a model for human skin disorders with dysregulated interferon immunity based on the comparative analysis.

Strengths

• Data is derived from controlled in vitro experiments using CRISPR-Cas9 and shRNA methods, as described.
• Includes comparative analysis between human and canine models, a key feature mentioned.
• The dataset is 3.7 MB in size, suggesting a focused and manageable scope for analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The data is contained within a PDF file, which may require extraction to be used computationally.

Provenance

Source

Sarah Quinlan

Collection Method

In vitro stimulation of keratinocytes with dsDNA/dsRNA ligands, followed by RT-qPCR analysis and genetic manipulation via CRISPR-Cas9 and shRNA.

Freshness

Last updated 2026-05-15 04:28:10; freshness should be verified.