Bovine Sperm Proteomics Before and After Cryopreservation from 5 Bulls

1,373 proteins were identified from semen samples of five sexually mature bulls, with 84 showing significant differential abundance after cryopreservation. The dataset, created by Alexandra Keller and last updated in 2026, results from a bottom-up proteomic LC-MS/MS analysis comparing fresh and cryopreserved sperm. Functional enrichment suggests proteins associated with metabolic processes, keratinization, reproductive processing, and cytoskeletal organization are affected.

Use Cases

• Train classification models to predict sperm cryosurvival based on proteomic profiles.
• Identify protein interaction networks disrupted by cryopreservation based on the 84 differentially abundant proteins.
• Validate biomarkers for mitochondrial integrity and redox homeostasis mentioned in the description.
• Correlate protein abundance shifts with functional annotations like cytoskeletal organization and reproductive processing.

Strengths

• Includes 1,373 identified proteins after filtering.
• Analysis is based on samples from five bulls with proven fertility.
• Differential abundance analysis identified 84 proteins with a log2FC > 2 and p < 0.05.
• Released under a permissive CC-BY-4.0 license.

Limitations

• The dataset is small at 834.7 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Bottom-up proteomic analysis using LC-MS/MS on divided semen samples.

Time Range

The study period is not specified.

Freshness

Last updated 2026-04-16 05:26:11; freshness should be verified.

Geography

The geographic origin of the bulls is not specified.