Data Sheet 1_Comparative proteomic analysis of colorectal cancer stem cells reveals potent

A proteomic dataset from 2026 by Ola J. Hussein, comparing colorectal cancer stem cells (CSCs) from two cell lines (SW620 and HCT-116) with their parental adherent cells. The 101.2 KB Excel file contains results from mass spectrometry-based label-free shotgun proteomics and subsequent pathway analysis. It highlights differentially abundant proteins and dysregulated pathways related to stemness, metabolism, and immune modulation.

Use Cases

• Identify potential protein biomarkers for colorectal cancer stem cells based on differentially abundant proteins.
• Analyze dysregulated canonical pathways, such as metabolic reprogramming and TGF-β signaling, in cancer stem cells.
• Validate predicted upstream regulators like MYC, MLXIPL, EGF/EGFR, VEGFA, and HIF-related signaling in stem cell models.
• Investigate the role of specific proteins like HMGCS1 in the mevalonate pathway and its association with therapeutic resistance.

Strengths

• Data is derived from a comparative proteomic study using mass spectrometry on two established colorectal cancer cell lines.
• Includes validation of selected findings via RT-qPCR and/or Western blotting, as mentioned in the description.
• Released under a permissive CC-BY-4.0 license, facilitating reuse and sharing.

Limitations

• Row count and column-level documentation are unknown, which limits suitability assessment and requires manual inspection.
• The dataset is small (101.2 KB), indicating limited scope, likely containing summary results rather than raw proteomic spectra.
• Data is based on in vitro cell line models (SW620 and HCT-116), which may not fully represent in vivo tumor heterogeneity.

Provenance

Source

figshare, author Ola J. Hussein.

Collection Method

Proteomic profiling via mass spectrometry-based label-free shotgun proteomics on CSC-enriched spheroids and parental adherent cells, analyzed with Ingenuity Pathway Analysis (IPA).

Freshness

Last updated 2026-04-20 04:13:39; freshness should be verified.