TCGA-READ Rectal Cancer Data for Alternative Splicing and Immune Subtype Analysis

217 potential tumor antigen genes were identified from TCGA-READ data, with FAM135A highlighted for its correlation with immune cell infiltration. The dataset, a 51.1 KB PDF published by Jiawen Weng in April 2026, stratifies patients into C1 and C2 immune subtypes to guide mRNA vaccine design and immunotherapy. Analysis identified 4480 upregulated and 3328 downregulated alternative splicing events, with exon skipping being the most common.

Use Cases

• Identifying potential mRNA vaccine antigens based on upregulated alternative splicing anomalies and frameshift mutations.
• Stratifying rectal cancer patients into immune subtypes (C1/C2) to predict response to CTLA-4/PD-1 blockade therapy.
• Analyzing correlations between specific antigen genes like FAM135A and immune cell infiltration profiles.
• Profiling the frequency and types of alternative splicing events, such as exon skipping and missense mutations, in rectal adenocarcinoma.

Strengths

• Identifies 217 potential antigen genes and 4480 upregulated alternative splicing events, providing specific molecular targets.
• Stratifies TCGA-READ samples into C1 and C2 subtypes with distinct immunotherapy benefits, offering clinical relevance.
• Published under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is a 51.1 KB PDF, suggesting it likely contains summarized analysis results rather than raw, large-scale data.
• Description metadata is limited; actual data quality and structure require manual inspection of the PDF file.

Provenance

Source

figshare, author Jiawen Weng.

Collection Method

Utilized public data (TCGA-READ) and bioinformatics analysis.

Time Range

Temporal coverage of the underlying TCGA-READ data is not specified.

Freshness

Last updated 2026-04-13 04:35:31; freshness should be verified.

Geography

Spatial coverage is not specified.