CRISPR-Cas9 Editing Results for Kinetochore Protein Phosphosites in Leishmania Mexicana

Data Sheet 1 details a precision editing study using CRISPR-Cas9 to modify phosphosite codons in kinetochore proteins of Leishmania mexicana. The work, authored by Charlotte McNiven and last updated in April 2026, reports a 27.5% successful editing rate from 18 transfections, with 30.4% of clones confirmed as edited and 22.1% of those being homozygous. The dataset includes a Python script for automating the design of 160 bp DNA repair templates.

Use Cases

• Benchmarking CRISPR-Cas9 editing efficiency in Leishmania based on reported 27.5% success rate.
• Analyzing the functional impact of phosphosite mutations on cell growth based on the description of generated mutants.
• Adapting the described Python script for automated repair template design in other kinetoplastid organisms.
• Studying kinetochore protein evolution and conservation based on the targeted proteins KKT2, KKT4, and KKT7.

Strengths

• Provides specific editing metrics: 27.5% editing success rate from 18 transfections.
• Includes a developed Python script for automating the design of 160 bp DNA repair templates.
• Focuses on six specific phosphosites across three essential kinetochore proteins (KKT2, KKT4, KKT7).

Limitations

• Row count and column-level documentation are absent; data structure must be inferred from the DOCX file.
• The dataset is small at 454.4 KB, indicating limited scope.
• Data may reflect experimental bias inherent to the specific lab conditions and Leishmania mexicana promastigotes.

Provenance

Source

Charlotte McNiven via figshare.

Collection Method

CRISPR-Cas9 genome editing with 160-bp double-stranded DNA repair templates, followed by PCR screening and Sanger sequencing.

Time Range

Experimental timeframe not specified; dataset updated April 2026.

Freshness

Last updated 2026-04-14 05:12:02.

Geography

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