School Wastewater Surveillance of Carbapenemase Genes in Santiago, Chile (2024)

A dataset from a 2024 study integrating culture, qPCR, and whole-genome sequencing to monitor blaKPC, blaNDM, and blaVIM carbapenemase genes in school wastewater in Santiago, Chile. The data includes seasonal composite samples, bacterial isolate counts, resistance rates, and absolute gene copy numbers. It was authored by Andrés Cortez-Astorga and shared under a CC-BY-4.0 license.

Use Cases

• Modeling seasonal dynamics of antimicrobial resistance based on quantified gene copy numbers across four seasons.
• Analyzing bacterial community composition and resistomes based on whole-genome sequencing of isolates like Aeromonas and Pseudomonas.
• Correlating culture-based isolation rates with direct molecular quantification from wastewater DNA.
• Investigating co-carriage of multiple carbapenemase genes and critical-priority sequence types like Escherichia coli ST1193.

Strengths

• Integrates three distinct methodologies: culture-based isolation, absolute qPCR, and whole-genome sequencing.
• Provides seasonal resolution with four 8-hour composite samples collected in 2024.
• Includes specific quantified results, such as resistance rates (e.g., 11.1% ± 1.72 for ciprofloxacin in winter) and gene copy numbers (up to 10^7 for blaKPC).
• Identifies specific bacterial genera (e.g., 43 Aeromonas isolates) and critical genotypes like mcr-1 and mcr-3.17.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset's scale is limited, derived from a single educational establishment in one city over one year.
• Description metadata is limited; actual data quality and structure require manual inspection of the PDF file.

Provenance

Source

figshare, authored by Andrés Cortez-Astorga.

Collection Method

Culture-based isolation, absolute qPCR, and whole-genome sequencing of wastewater samples.

Time Range

2024, with samples from four seasons.

Freshness

Last updated 2026-04-23 05:35:01; freshness should be verified.

Geography

Santiago, Chile, specifically from an educational establishment.