gpEvolvR: Design and Validation Data for a Curable Directed Evolution System

A 13.0 MB document authored by Paul M. Will, last updated on 2026-05-26, describes the design and validation of a modified EvolvR plasmid system for targeted hypermutation. The work demonstrates functionality in both Escherichia coli and Corynebacterium glutamicum, including mutagenesis of a plasmid origin and a genomic β-carotene ketolase (CrtW) gene. The dataset is available under a CC-BY-4.0 license on figshare.

Use Cases

• Benchmarking new directed evolution tools based on the described plasmid curing and mutagenesis features.
• Designing plate-based screening assays for enzyme variants based on the β-carotene ketolase (CrtW) evolution example.
• Studying the transferability of mutagenesis systems between Escherichia coli and Corynebacterium glutamicum based on the cross-species functionality described.

Strengths

• Documented proof-of-principle results, including specific kanamycin minimal inhibitory concentration increases of +44% and +96% in E. coli mutants.
• Describes a complete, curable plasmid system (gpEvolvR) with features for post-mutagenesis removal.
• Provides a concrete application example by targeting the β-carotene ketolase (CrtW) gene in C. glutamicum.

Limitations

• The dataset is a 13.0 MB DOCX file; the underlying experimental data (e.g., sequence files, raw screening results) are not explicitly described or available.
• Row and column structures are unknown, limiting immediate machine-readability and analysis.
• The description is specific to a proof-of-concept study; the scale and diversity of generated mutant libraries are not quantified.

Provenance

Source

figshare

Collection Method

Experimental research involving plasmid construction, bacterial transformation, and phenotypic screening.

Freshness

Last updated 2026-05-26 09:20:35.