Differential Spleen Immune Signatures in Murine Scrub Typhus Infection

A research document by Casey Gonzales, uploaded to figshare in April 2026, compares host immune responses to two strains of Orientia tsutsugamushi in a mouse model. The study provides flow cytometry, immunohistology, serological, and RNA sequencing data from spleen samples during acute infection.

Use Cases

• Compare B cell and germinal center responses based on flow cytometry and immunohistology data mentioned in the description
• Analyze strain-specific inflammatory gene expression pathways based on RNA sequencing profiles
• Correlate bacterial burden and mortality outcomes with innate immune cell influx based on the described experimental results

Strengths

• Provides a direct comparison of two clinically relevant bacterial strains (Karp and Gilliam)
• Includes multi-modal analysis: flow cytometry, immunohistology, serology, and RNA sequencing
• Reports specific quantitative outcomes, such as a 50% mortality rate for the Karp strain

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is a 1.0 MB DOCX file, which is a small document format rather than a structured data table

Provenance

Source

Casey Gonzales via figshare

Collection Method

Experimental data from a C57BL/6 murine model using equivalent infectious doses

Time Range

Acute infection phase (day 4 post-infection mentioned)

Freshness

Last updated 2026-04-23 04:20:04; freshness should be verified

Geography

Laboratory study; geographic origin of data not specified