ant(9)-Ie: Characterization of a Novel Spectinomycin Resistance Gene in Providencia

A 550 KB PDF describes the discovery and characterization of a novel chromosomally encoded aminoglycoside resistance gene, ant(9)-Ie, in Providencia rettgeri P17. The research includes functional validation via molecular cloning, showing a 64-fold increase in spectinomycin MIC, and kinetic analysis of the ANT(9)-Ie enzyme. The dataset, authored by Lulu Huang and last updated in June 2026, is a CC-BY-4.0 licensed research article from figshare.

Use Cases

• Study the genetic environment and phylogeny of ant(9)-Ie based on comparative genomic analysis described.
• Analyze the kinetic parameters of the ANT(9)-Ie enzyme based on the reported catalytic efficiency (kcat/Km).
• Investigate spectinomycin resistance profiles in bacterial isolates based on the Minimum Inhibitory Concentration (MIC) data.
• Examine the distribution of ant(9)-Ie homologous genes across Providencia strains based on the conserved genetic environment mentioned.

Strengths

• Includes specific kinetic parameter for ANT(9)-Ie enzyme: (8.22 ± 1.24) × 10² M⁻¹·s⁻¹.
• Reports a 64-fold increase in spectinomycin Minimum Inhibitory Concentration (MIC) for the recombinant strain.
• Provides a comparative analysis showing 58.67% amino acid sequence similarity to ANT(9)-Id.

Limitations

• Dataset is a 550 KB PDF; the underlying raw genomic or experimental data is not directly provided.
• Row count and column-level documentation are unknown, limiting suitability assessment for computational reuse.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, authored by Lulu Huang.

Collection Method

Bacteria isolated from wastewater from an animal farm; characterized using whole-genome sequencing, molecular cloning, and kinetic analysis.

Freshness

Last updated 2026-06-02 04:28:46; freshness should be verified.