Breast Milk and Infant Gut Microbiota and SCFAs from Rural and Urban Dyads in Manitoba

Infant stool samples from 0-12 months and breast milk samples from 1-30 days postpartum were collected from 69 mother-infant dyads in rural and urban Manitoba. The dataset, authored by Saeid Khakisahneh and last updated in May 2026, includes results from 16S rRNA sequencing and gas chromatography–mass spectrometry analyses of microbiota and short-chain fatty acids. It reveals distinct microbial patterns and metabolite levels associated with geographical residency.

Use Cases

• Compare infant gut microbiota maturity trajectories based on rural versus urban residency.
• Analyze correlations of specific genera like Alistipes and Blautia between breast milk and infant stool.
• Investigate associations between short-chain fatty acid levels (e.g., acetic, valeric, isobutyric acid) and geographical location.
• Study the impact of maternal gestational diabetes on milk and infant gut microbiota within the cohort.

Strengths

• Includes data from 69 mother-infant dyads, providing a paired sample structure.
• Combines 16S rRNA sequencing for microbiota with gas chromatography–mass spectrometry for metabolite (SCFA) profiling.
• Contains samples from two distinct geographical settings (rural and urban Manitoba) and includes 10 mothers with gestational diabetes.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The dataset is relatively small at 1.8 MB, which may constrain statistical power for some analyses.
• Data is provided in a DOCX file format, which may require extraction and structuring before computational analysis.

Provenance

Source

figshare

Collection Method

Samples were collected and analyzed using 16S rRNA sequencing and gas chromatography–mass spectrometry.

Time Range

Infant stool: 0-12 months; Breast milk: 1-30 days postpartum.

Freshness

Last updated 2026-05-29 05:33:16; freshness should be verified.

Geography

Manitoba, Canada (rural and urban areas).