Data Sheet 4: PD-L1 Knockout Mouse Data on Shock/Sepsis Lung Injury

Elizabeth W. Tindal's experimental data compares the impact of PD-L1 deficiency in endothelial cells versus neutrophils on mortality and lung injury in a murine hemorrhagic shock and sepsis model. Fourteen-day survival and lung permeability data, alongside cytokine, chemokine, and angiopoietin levels from blood, BALF, and lung tissue, are included. The dataset supports investigation into cell-type-specific immune checkpoint roles in critical illness.

Use Cases

• Comparing mortality outcomes based on cell-specific PD-L1 knockout status.
• Analyzing lung vascular permeability changes using Evan's Blue dye exclusion assay results.
• Correlating cytokine/chemokine levels in lung tissue, BALF, and blood with organ injury indices.
• Validating flow cytometry gating strategies for immune cell analysis in shock/sepsis models.

Strengths

• Data is derived from controlled Cre-lox mouse models allowing cell-type-specific (endothelial vs. neutrophil) genetic analysis.
• Includes multiple experimental readouts: survival, flow cytometry, ELISA for cytokines/chemokines/angiopoietins, and organ injury assays.
• Published under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• Column names and exact row counts are not provided, limiting immediate understanding of data structure.
• The dataset appears to be a single PDF document (Data Sheet 4), which may require extraction for computational analysis.
• All metadata entries list 'Rows: None' or 'Rows: unknown', indicating incomplete technical documentation.

Provenance

Source

Elizabeth W. Tindal

Collection Method

Data generated from laboratory experiments using adult male C57BL/6 mice subjected to hemorrhagic shock and cecal ligation and puncture (CLP) or sham procedures.

Freshness

Last updated on 2026-06-02.