CRISPR/Cas9-Edited Papaya Lines for PRSV Resistance

A 1.3 MB document details the creation of transgenic papaya T0 lines with edited eIF4E and eIF(iso)4E genes via CRISPR/Cas9. Author Ngoc Thu Le published the work on figshare in May 2026. The study identifies eIF4E as a critical host factor for papaya ringspot virus, with edited lines showing undetectable viral accumulation via DAS-ELISA and qPCR.

Use Cases

• Training models to predict gene-editing outcomes based on mutation patterns described in the study.
• Analyzing the differential role of eIF4E versus eIF(iso)4E in potyvirus resistance mechanisms.
• Serving as foundational germplasm data for breeding programs aiming to develop PRSV-resistant papaya cultivars.

Strengths

• Includes specific experimental validation methods: DAS-ELISA and qPCR confirmed undetectable viral accumulation in resistant lines.
• Documents a clear causal finding: only eIF4E-edited plants demonstrated resistance across all tested time points.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• The primary data file is a 1.3 MB DOCX document; underlying raw sequencing or phenotypic data may not be included.
• Row count and column-level documentation are unknown, limiting suitability assessment for computational analysis.
• The study notes a limitation in using chimeric T0 mutant lines for immediate breeding.

Provenance

Source

figshare

Collection Method

Data generated via Agrobacterium-mediated transformation of somatic embryos and analyzed via heteroduplex and Sanger sequencing.

Freshness

Last updated 2026-05-19 05:38:34.