Environmental Resistome of Gram-Negative Bacilli from the Yaque del Norte River

A 2026 study by María F. Chevalier-Alba provides genomic evidence for antimicrobial resistance surveillance. It characterizes the resistome of six culturable Gram-negative bacterial strains isolated from the Yaque del Norte River in the Dominican Republic. Whole-genome sequencing identified 112 antimicrobial resistance genes, with Pseudomonas aeruginosa harboring 51 genes.

Use Cases

• Baseline analysis of antimicrobial resistance gene diversity in a freshwater ecosystem based on the identified 112 ARGs.
• Studying potential interspecies plasmid dissemination based on the detection of IncY and IncFIB(K) replicons in Klebsiella species.
• Evaluating the pathogenic potential of environmental isolates using the PathogenFinder tool mentioned in the description.
• Supporting antibiotic stewardship and surveillance strategies in the Dominican Republic based on the genomic evidence provided.

Strengths

• Identifies 112 specific antimicrobial resistance genes across six bacterial strains.
• Provides whole-genome sequencing data analyzed with specific bioinformatic tools (CARD, Galaxy staramr, PathogenFinder).
• Includes phenotypic resistance data for a Klebsiella pneumoniae isolate.

Limitations

• The dataset is very small in scale (167.4 KB PDF), containing genomic evidence from only six bacterial isolates.
• Column-level documentation is absent; field semantics must be inferred from the PDF report.
• Data may reflect geographic bias inherent to a single river basin in the Dominican Republic.

Provenance

Source

figshare, authored by María F. Chevalier-Alba.

Collection Method

Water samples were collected along the river basin. Bacterial isolates were identified using MALDI-TOF MS and JspeciesWS, followed by whole-genome sequencing and bioinformatic analysis.

Freshness

Last updated 2026-04-21 05:34:58; freshness should be verified.

Geography

Yaque del Norte River, Dominican Republic.