Data Sheet 1: SIRT3 Ligand Binding and Cardiac Cell Assay Results

Data Sheet 1 contains experimental results from a study on the interaction between the synthetic fatty acid analog SA-22 and the mitochondrial protein SIRT3. The dataset, authored by Joshua W. Kranrod and last updated in June 2026, includes measurements from thermal shift assays, catalytic activity assays, and cell-based models of hypoxia/reoxygenation injury in H9c2 cardiac cells. It is shared under a CC-BY-4.0 license via figshare.

Use Cases

• Train models to predict protein-ligand interactions based on thermal shift assay data.
• Analyze the impact of pharmacological modulators on mitochondrial respiration parameters.
• Study the relationship between mitophagy and cellular injury in a controlled in vitro model.

Strengths

• Includes data from multiple experimental modalities: thermal shift assays, fluorogenic activity assays, and high-resolution respirometry.
• Experimental conditions and inhibitor concentrations (e.g., 1 µM SA-22, 50 µM 3-TYP) are explicitly documented in the description.
• The dataset is associated with a peer-reviewed research methodology described in the background and methodology sections.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale statistical modeling.
• The 5.4 MB file size suggests a relatively small dataset.

Provenance

Source

Joshua W. Kranrod via figshare

Collection Method

Experimental data from in vitro biochemical assays and cell culture models.

Freshness

Last updated 2026-06-02 04:21:32; freshness should be verified.