TRV-VIGS System in Coix: Experimental Data for Functional Genomics

A 787.2 KB PDF document published on figshare by Xiaosheng Zhao on April 14, 2026. It describes the establishment of a virus-induced gene silencing system for the cereal crop Coix lacryma-jobi, including experimental protocols and results for studying smut disease resistance.

Use Cases

• Optimizing gene silencing protocols based on described vacuum infiltration parameters and methylglyoxal supplementation.
• Studying plant defense mechanisms based on the silencing of the host regulator ClNPR1 mentioned in the description.
• Developing host-induced gene silencing strategies based on the described silencing of the pathogen effector UcPep1.
• Evaluating smut disease resistance in Coix based on the described Ustilago coicis inoculation protocol.

Strengths

• Provides a first-reported VIGS system for Coix, a previously understudied crop.
• Includes specific optimization results, such as boosting silencing efficiency from 25% to 57% with 200 μM methylglyoxal.
• Validates the system with concrete examples, silencing both a host gene (ClNPR1) and a pathogen gene (UcPep1).

Limitations

• The dataset is a 787.2 KB PDF; raw experimental data tables or sequences are not directly provided.
• Column-level documentation is absent; field semantics must be inferred from the manuscript text.
• Row count is unknown, which may limit suitability assessment for direct computational analysis.

Provenance

Source

figshare

Collection Method

Experimental research described in a scientific manuscript.

Time Range

The study period is not specified.

Freshness

Last updated 2026-04-14 05:38:29.

Geography

The geographic origin of the Coix samples or experiments is not specified.