Evidence on Tyrosine Hydroxylase Activity in Mouse Supraoptic Nucleus Neurons

A research document investigating the enzymatic activity of tyrosine hydroxylase (TH) in neurons of the mouse supraoptic nucleus. The study used 77 male C57BL/6 mice, applying immunohistochemistry, laser microdissection, PCR, and chromatography. The author, Tatiana S. Pronina, published the findings on figshare in April 2026.

Use Cases

• Analyzing gene and protein expression patterns for tyrosine hydroxylase based on the described immunohistochemistry and PCR methods.
• Studying the relationship between osmotic stress and neuronal markers based on the described salt-loading experimental design.
• Investigating the role of enzyme cofactors like tetrahydrobiopterin based on the described analysis of guanosine triphosphate cyclohydrolase 1 expression.
• Comparing protein structure motifs (e.g., N-terminus) between different neuron types based on the described findings in the substantia nigra versus the supraoptic nucleus.

Strengths

• Provides a detailed methodological description including immunohistochemistry, laser microdissection, PCR, Western blotting, and HPLC.
• Includes experimental data from 77 mice across three conditions (intact, 0.9% NaCl, 8.5% NaCl).
• Released under a permissive CC-BY-4.0 license, allowing for reuse and redistribution.

Limitations

• The dataset is a 920.9 KB DOCX file; the underlying raw data tables or measurements are not directly accessible in a structured format.
• Row count and column-level documentation are unknown, requiring manual inspection after download to assess data structure.
• The description is specific to a single, focused biological hypothesis, which limits its general applicability.

Provenance

Source

figshare, authored by Tatiana S. Pronina.

Collection Method

Experimental study using mouse models, laser microdissection, polymerase chain reaction, Western blotting, and high-performance liquid chromatography.

Time Range

Study likely conducted on 8–10 week old mice; specific collection dates are not provided.

Freshness

Last updated 2026-04-28 04:28:53; freshness should be verified.

Geography

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