Ex Vivo Nasal Epithelial Cytokine Response to HRV and Influenza Infection

A research dataset from figshare, authored by Leila Fotoohabadi and last updated in June 2026. It contains measurements from a polarized ex vivo culture system of primary human nasal epithelial cells infected with human rhinovirus (HRV16) or influenza virus (H1N1-PR8). The data includes cytokine secretion levels for TNF-α, VEGF, IL-1β, IL-6, IL-8, and IL-10 measured at apical and basal surfaces over 24 and 48 hours.

Use Cases

• Compare inflammatory cytokine profiles based on infection with HRV16 versus H1N1-PR8.
• Analyze differential secretion patterns based on apical versus basal cytokine measurements mentioned in the description.
• Investigate the role of VEGF in virus-mediated nasal congestion based on the described upregulation.
• Model early nasal epithelial immune responses based on the described ex vivo air-liquid interface culture system.

Strengths

• Data is derived from a novel polarized ex vivo culture system, providing a physiologically relevant model.
• Measures cytokine levels for multiple targets (TNF-α, VEGF, IL-1β, IL-6, IL-8, IL-10) at two time points (24h, 48h).
• Licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• The dataset is very small at 65.0 KB, indicating limited scope.
• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The description does not specify the number of biological replicates or donor heterogeneity.

Provenance

Source

figshare, author Leila Fotoohabadi.

Collection Method

Data generated from a polarized ex vivo culture system of primary, immortalized human nasal epithelial cells (hNECs) in air-liquid interface (ALI) culture.

Freshness

Last updated 2026-06-03 06:01:06; freshness should be verified.