Cryptosporidium Rhomboid Protease Phylogeny and Sporozoite Localization

Ilaria Vanni's 2026 figshare publication analyzes three rhomboid proteases (CpRom1, CpRom2, CpRom3) in the Cryptosporidium parvum parasite. The study details their phylogenetic origin, structural motifs, and distinct subcellular localization within sporozoite cells. It also lists 10 candidate membrane protein substrates for these proteases.

Use Cases

• Phylogenetic classification of CpRom1, CpRom2, and CpRom3 based on catalytic site similarities to Toxoplasma gondii and Plasmodium falciparum orthologs.
• Analysis of subcellular localization patterns: CpRom1 at the apical complex and posterior pole, CpRom2 in the apical complex, and CpRom3 along the entire sporozoite surface.
• Identification of 10 candidate substrate membrane proteins for C. parvum rhomboids based on similarity to known apicomplexan substrates and co-localization evidence.
• Comparative genomic analysis of PARL-like rhomboid gene presence in 'intestinal' versus 'gastric' Cryptosporidium species.

Strengths

• Provides detailed structural and phylogenetic analysis for three specific rhomboid proteases (CpRom1, CpRom2, CpRom3).
• Includes specific subcellular localization data for each protease within sporozoite cells.
• Identifies 10 candidate substrate proteins based on defined similarity and co-presence criteria.

Limitations

• The dataset is a single 204.8 KB PDF document, containing textual analysis rather than structured, machine-readable data tables.
• No raw experimental data (e.g., sequence alignments, microscopy images, quantitative measurements) is provided for independent computational analysis.
• The scope is limited to Cryptosporidium parvum and a comparative analysis with a few other apicomplexan species.

Provenance

Source

figshare, authored by Ilaria Vanni.

Collection Method

Bioinformatic and experimental analysis including phylogenetic studies, structural motif examination, and cellular localization techniques.

Time Range

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Freshness

Last updated April 7, 2026.

Geography

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