Monoclonal Antibody 4H3 Specific to HN Protein of PPMV-1

Xu Wang's research document details the generation and characterization of monoclonal antibody 4H3 targeting the HN protein of pigeon paramyxovirus type 1 (PPMV-1). The study includes codon optimization, prokaryotic expression, antibody screening, and epitope mapping identifying the conserved DRVWF epitope. The dataset was last updated on April 24, 2026.

Use Cases

• Design PPMV-1-specific diagnostic assays based on the identified monoclonal antibody.
• Investigate HN protein function in viral adsorption and egress based on the described epitope.
• Develop epitope-based vaccines against pigeon Newcastle disease based on the conserved DRVWF sequence.
• Screen for cross-reactivity with other NDV strains based on the described Western blot and IFA results.

Strengths

• The monoclonal antibody 4H3 is confirmed to specifically react with PPMV-1 strains.
• The minimal B-cell epitope is precisely identified as amino acids 310-314 (DRVWF).
• The epitope is shown to be highly conserved across PPMV-1 strains through sequence homology analysis.

Limitations

• Dataset is a 1.5 MB DOCX file; the underlying data structure and column-level documentation are absent.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.
• Freshness should be verified as the last update date is in the future (2026-04-24).

Provenance

Source

figshare

Collection Method

Research study involving codon optimization, prokaryotic expression, cell fusion, antibody screening, and epitope mapping.

Freshness

Last updated 2026-04-24 05:36:47